Two mutations associated with macrolide resistance in Treponema pallidum in Shandong, China.
نویسندگان
چکیده
Zhen Li, Jianling Hou, Rongtao Zheng, Zhongwei Li, Jing Wen, Dianchang Liu, Ruyuan Liu, Tongsheng Chu, Bing Liu, Gongqi Yu, Hongqing Tian, FuRen Zhang Shandong Provincial Institute of Dermatology and Venereology, Provincial Academy of Medical Science, Jinan, Shandong, China; Shandong Provincial Hospital for Skin Diseases, Jinan, Shandong, China; Shandong Provincial Medical Center for Dermatovenereology, Jinan, Shandong, China; Shandong Provincial Key Lab for Dermatovenereology, Jinan, Shandong, China
منابع مشابه
High prevalence of macrolide resistant Treponema pallidum strains in a London centre.
OBJECTIVES Macrolide resistant Treponema pallidum strains, caused by mutations in the 23S ribosomal RNA (23S rRNA) gene, are widespread and increasingly prevalent. The authors aimed to establish the strain types of T pallidum isolated from patients in a London sexual health centre and to determine the frequency of macrolide resistance. METHODS T pallidum DNA from blood and ulcer samples were ...
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Macrolide treatment failure in syphilis patients is associated with a single point mutation (either A2058G or A2059G) in both copies of the 23S rRNA gene in Treponema pallidum strains. The conventional method for the detection of both point mutations uses nested PCR combined with restriction enzyme digestions, which is laborious and time-consuming. We initially developed a TaqMan-based real-tim...
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BACKGROUND High rates of 23S rDNA mutations implicated in macrolide resistance have been identified in Treponema pallidum samples from syphilis patients in many countries. Nonetheless, some clinicians have been reluctant to abandon azithromycin as a treatment for syphilis, citing the lack of a causal association between these mutations and clinical evidence of drug resistance. Although azithrom...
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A total of 54 clinical samples, including genital lesion swabs, whole blood and cerebrospinal fluid from patients diagnosed with syphilis were collected in 2006 and in 2013 in Buenos Aires, Argentina. Treponemal DNA was detected in 43 of the analyzed samples (79.6%) and further analyzed using Sequencing-based molecular typing (SBMT) and Enhanced CDC-typing (ECDCT). By SBMT, 10 different Trepone...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 51 12 شماره
صفحات -
تاریخ انتشار 2013